Hematological parameters in rainbow trout, Oncorhynchus mykiss, after exposure to ibuprofen

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Ibuprofen, Rainbow trout, Haematological parameters


Ibuprofen, a non-steroidal anti-inflammatory drug, has been found in the aquatic environment, but its effects on fish and other aquatic organisms remain largely unknown. The aim of this study was to investigate the effect of Ibuprofen on haematological parameters in rainbow trout (Oncorhynchus mykiss). Fish were exposed to immersion two different doses of ibuprofen concentrations (50 and 500 µg/L) for 96 hours. Exposure to acute toxicity resulted in abnormal behavior of some fish. In this study, a significant decrease in red blood cells (RBC) and mean cellular hemoglobin concentration (MCHC) were observed throughout the study period. Hematocrit (Hct), Haemoglobin (Hb), white blood cells (WBC), mean cellular volume (MCV) and mean cellular hemoglobin (MCH) levels were increased. This study demonstrated that different doses of Ibuprofen were associated with different toxic effects on the hematological parameters in rainbow trout.


Al-Rudainy, A. J. (2015). Effects of sub-lethal exposure to lead acetate on haematological indices and growth rate of Bunni Mesopotamichthys sharpeyi. Advances in Animal and Veterinary Sciences, 3(11), 569-573.

Ascar, L., Ahumada, I., López, A., Quintanilla, F., & Leiva, K. (2013). Nonsteroidal anti-inflammatory drug determination in water samples by HPLC-DAD under isocratic conditions. Journal of the Brazilian Chemical Society, 24(7), 1160-1166.

Burke, A., Smyth, E. M., & Fitzgerald, G. A. (2006). Analgesic-antipyretic agents; pharmacotherapy of gout, in Goodman and Gilman’s, The pharmacologic basis of therapeutics. In: Brunton, L. L., Laxo, J. S., & Parker, K. L. (Eds.), 11th ed. McGrawHill, New York.

Drabkin, D. L. (1946). Spectrometric studies, XIV: the crystallographic and optimal properties of the hemoglobin of man in comparison with those of other species. Journal of Biological Chemistry, 164,703-723.

Green, G. A. (2001). Understanding NSAIDs: from aspirin to COX-2. Clinical Cornerstone, 3(5),50–59.

Härdig, J., & Höglund, L. B., (1983). On accuracy in estimating fish blood variables. Comparative Biochemistry and Physiology Part A: Physiology, 75(1), 35-40.

Kohler, H. R., Sandu, C., Scheil, V., Nagy-Petrica, E. M., Segner, H., & Telcean, I. (2007). Monitoring pollution in River Mures, Romania, Part 111: Biochemical effects markers in fish and integrative reflection. Environmental Monitoring and Assessment,127, 47-54.

Li, Z. H., Velisek, J., Zlabek, V., Grabic, R., Machova, J., Kolarova, J., & Randak, T. (2010). Hepatic antioxidant status and hematological parameters in rainbow trout, Oncorhynchus mykiss, after chronic exposure to carbamazepine. Chemico-Biological Interactions, 183(1), 98-104.

Li, Z. H., Zlabek, V., Velisek, J., Grabic, R., Machova, J., Kolarova, J., Li, P., & Randak, T. (2011). Acute toxicity of carbamazepine to juvenile rainbow trout (Oncorhynchus mykiss): effects on antioxidant responses, hematological parameters and hepatic EROD. Ecotoxicology and Environmental Safety, 74(3), 319-327.

Natt, M. P., & Herrick, C. A. (1952). A new blood diluent for counting the erythrocytes and leucocytes of the chicken. Pollution Science, 31, 735-738.

Nussey, G., Vuren, J. H. V., & Preez, H. H. D. (2002). The effect of copper and zinc at neutral and acidic pH on the general haematology and osmoregulation of Oreochromis mossambicus. African Journal of Aquatic Science, 27, 61–84.

Nussey, G., Van Vuren, J. H. J., & Du Preez, H. H. (1995). Effect of copper on the differential white blood cell counts of the Mozambique tilapia (Oreochromis mossambicus). Comparative Biochemistry and Physiology, 111, 381-388.

Oliveira Ribeiro, C. A., Belger, L., Pelletier, E., & Rouleau, C., (2002). Histopathological evidence of inorganic mercury and methyl mercury toxicity in the arctic charr (Salvelinus alpines). Environmental Research, 90, 217–225.

Pamplona, J. H., Oba, E. T., Da Silva, T. A., Ramos, L. P., Ramsdorf, W. A., Cestari, M. M., Oliveira Ribeiro, C.A., Zampronio, A.R., & Assis, H. S. (2011). Subchronic effects of dipyrone on the fish species Rhamdia quelen. Ecotoxicology and Environmental Safety,74(3), 342-349.

Pluciennik-Koropczuk, E. (2014). Non-Steroid Anti-Infflamatory Drugs in Municipal Wastewater and Surface Waters. Civil and Environmental Engineering Reports, 14(1), 63-74.

Ribas, J. L. C., Zampronio, A. R., & Silva de Assis, H. C. (2015). Effects of trophic exposure to diclofenac and dexamethasone on hematological parameters and immune response in freshwater fish. Environmental Toxicology and Chemistry, 35, 975–982.

Richards, N. L., Cook, G., Simpson, V., Hall, S., Harrison, N., & Scott, K. S. (2011). Qualitative detection of the NSAIDs diclofenac and ibuprofen in the hair of Eurasian otters (Lutra lutra) occupying UK waterways with GC–MS. European Journal of Wildlife Research, 57(5), 1107-1114.

Saravanan, M., Devi, K. U., Malarvizhi, A., & Ramesh, M. (2012). Effects of Ibuprofen on hematological, biochemical and enzymological parameters of blood in an Indian major carp, Cirrhinus mrigala. Environmental Toxicology and Pharmacology, 34(1), 14-22.

Saravanan, M., Karthika, S., Malarvizhi, A., & Ramesh, M. (2011). Ecotoxicological impacts of clofibric acid and diclofenac in common carp (Cyprinus carpio) fingerlings: hematological, biochemical, ionoregulatory and enzymological responses. Journal of Hazardous Materials, 195, 188-194.

Steinbach, C., Burkina, V., Schmidt-Posthaus, H., Stara, A., Kolarova, J., Velisek, J., Randak, T., & Kroupova, H. K. (2016). Effect of the human therapeutic drug diltiazem on the haematological parameters, histology and selected enzymatic activities of rainbow trout Oncorhynchus mykiss. Chemosphere, 157, 57-64.

Tavares-Dias, M., & Moraes, F.R. (2006). Hematological parameters for the Brycon orbignyanus Valenciennes, 1850 (Osteichthyes: Characidae) intensively bred. Hidrobiológica, 16, 271-274.

Ternes, T. A. (1998). Occurrence of drugs in German sewage treatment plants and rivers. Water Research, 32(11), 3245-3260.

Thomas, K. V., & Hilton, M. J. (2004). The occurrence of selected human pharmaceutical compounds in UK estuaries. Marine Pollution Bulletin, 49, 436–444.

Wintrobe, M. M. (1934). Variations on the size and hemoglobin content of erythrocytes in the blood various vertebrates. Folia Haematologica, 51, 32-49.




How to Cite

İspir, Ünal, & Şahin, A. (2023). Hematological parameters in rainbow trout, Oncorhynchus mykiss, after exposure to ibuprofen. Hydrobiological Research, 1(1), 1–9. https://doi.org/10.5281/zenodo.8328941



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