Writing Rules

Manuscripts must be submitted to the journal electronically, only as a member of the "Make a Submission" section.

Hydrobiological Research publishes original articles, reviews, short communications, method reports, and letters to the editor that examine the biology of marine and freshwater resources. The journal is an international, blind peer-reviewed, and open-access journal and publishes articles in English in 2 volumes (January, July) per year.

*You can suggest at least two reviewer names (and mail addresses) to the editor in Comments to the Editor. If the editor deems it appropriate, he can send it to the suggested names.

Article Processing Charges (APC)

The journal does not charge any submission and publication fees. All articles can be downloaded FREE of charge.

Manuscripts Format

Please prepare your manuscript according to the template of the Journal

*Download “Manuscript Template

*Download “Cover Letter

*Download “Copyright Transfer Form

The manuscript file, cover letter, and copyright transfer form are required to be uploaded in submission. Each page must be numbered. There is no page limit in the manuscript.

All communication regarding the manuscript will be made with the corresponding author. If any problem occurs during or after the submission of the manuscript, you can report it to the "editor@hydrobiologicalresearch.com", "support@hydrobiologicalresearch.com" and "hydrobiologicalresearch@gmail.com" e-mail addresses.

ORCID: All manuscript submissions require an ORCID ID. Authors who do not have an ORCID ID may register from the following link: https://orcid.org.

Double-blind review

Manuscripts undergo double-blind review, whereby the names of the reviewers are not disclosed to the authors and vice versa. This is to allow unbiased decisions. At least two reviewers evaluate the manuscripts, and the Editor decides on acceptance, revision, or rejection. Manuscripts returned to authors for revision should be resubmitted within the deadline; otherwise, the manuscript may be considered withdrawn. The deadline for the resubmission of the revised manuscript could be extended upon request to the Editor.

Article Types

Research articles: The preparation of research articles should follow the journal’s structure or simply use the formatting template. For details, please refer to the Formatting template or Manuscript preparation.

Reviews, method reports, short communication, and letter to editor: There is no specific formatting for these types of articles.

Manuscript Preparation

Prepare your text using word-processing software and save it in “.doc” or “.docx” formats. Manuscripts must be structured in the following order;

Main text

  • Title
  • Author names and affiliations
  • Abstract
  • Keywords
  • ORCID ID for all authors
  • Corresponding author’s e-mail
  • Introduction
  • Material and Methods
  • Results (or Results and Discussion) 
  • Discussion (or Discussion and Conclusion)
  • Conclusion
    • Acknowledgments (if required)
    • Authors’ Contributions
    • Conflict of Interest
    • Statement on the Welfare of Animals
    • Statement of Human Rights
    • Data Availability Statement
  • References
  • Appendices (if any).


Concise and informative title. Avoid abbreviations and formulae (14-points font, Times New Roman, bolt, left-aligned, 1.0 spaced, the first letter of the first word capitalized, other words begin with lowercase)

Author Names and Affiliations:

The first name(s) and surname(s) of the author(s) (The corresponding author should be identified with an asterisk and footnote ((12-point font, bolt, Times New Roman, left-aligned)

All other footnotes (Author(s) affiliation address(es)) should be identified with a lower-case superscript letter. Author(s) affiliation address(es) (followed by institution, faculty/school, department, city with postcode, and country) of each author(s). (Times New Roman,center-aligned, 1.0-spaced with 10-point font)

(University or Institute, Faculty or School, Department, Postal Code-City, Country)


The abstract title should be in 10-point font, Times New Roman, and bolt.

The abstract should be confined to a single paragraph and between 100 and 400 words. The text should be in Times New Roman, 1.0-spaced with a 10-point font. References and abbreviations should be avoided.  Do not use any abbreviations and citations in the abstract.


Keywords provide a maximum of 5 keywords, 10-point font, and Times New Roman. The first letter must be capitalized. They should be written in alphabetical order. 


Name Surname1: https://orcid.org/0000-000x-xxxx-xxxx

Name Surname2: https://orcid.org/0000-000x-xxxx-xxxx

Name Surname3: https://orcid.org/0000-000x-xxxx-xxxx


The text should be written in Times New Roman, 1.0-spaced with a 10-point font. Please provide a concise introduction without subheadings. Use 2 cm margins on all sides. The text should be in double-column format. In particular, do not use to hyphenate words. The names of genera and species should be given in italics and, when first mentioned in the text, should be followed by the authority.

Each page must be numbered, and lines must be consecutively numbered from the start to the end of the manuscript. The first mention in the text of any taxon must be followed by its authority including the year. Use italics for emphasis. Use only SI (international system) units.

Use a period in decimal fractions (3.16 rather than 3,16).

Use " µg/L", not " µg L-1 ".

With space 2 h, 2 g, 2 m,

Without space 4°C, 4%

Materials and Methods:

This section should provide details of the information that may help other researchers to replicate the study.


Results should be clear and concise. All outputs and consequences of applied methods and analyses must be given here. The results section can be stand-alone or combined with the Discussion section, which is optional to the authors’ preference.


The discussion section can be stand-alone or combined with the Results section, which is optional to the authors’ preference. This section should explore the significance of the results of the study, not repeat them. The findings and results must be compared and discussed with available findings in the literature. It can also include recommendations or suggest application of the results for further research.


This section should explore the significance of the results of the study, not repeat them. It can also include recommendations or suggest application of the results for further research.

Acknowledgments and Funding (if required):

Anyone who contributed to the research or manuscript, but who is not a listed author, should be acknowledged (with their permission). Authors can acknowledge any people/institutions for their support during the research and specify funding institution with grant number.

Compliance with Ethical Standards:

The corresponding author will include a summary statement in the text of the manuscript in a separate section before the reference list. See below examples of disclosures:

Authors’ Contributions:

Please provide the contributions of the authors for the paper. Use the first letters of the names and surnames of the authors. See below for an example;

MK: Manuscript design, Field sampling, Draft checking.

NK: Writing, Draft checking, Reading, Editing.

MRT: Laboratory experiments, Statistical analyses.

All authors read and approved the final manuscript.

Conflict of Interest

Any existing conflict of interest should be given here. If no conflict exists, the authors should state:

Conflict of Interest: The authors declare that there is no conflict of interest.

Statement on the Welfare of Animals

If the animals used in the study;

The welfare of animals used for research must be respected. When reporting experiments on animals, authors should indicate the following statement:

Ethical approval: All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Or, for retrospective studies; a summary statement in the text of the manuscript should be included as follows:

Ethical approval: For this type of study, formal consent is not required.

Statement of Human Rights

When reporting studies that involve human participants, authors should include the following statement:

Ethical approval: The studies have been approved by the appropriate institutional and/or national research ethics committee and have been performed in accordance with the ethical standards.

Or, for retrospective studies; a summary statement in the text of the manuscript should be included as follow:

Ethical approval: For this type of study, formal consent is not required.

Data Availability Statements:

Data Availability Statements should be placed in the back matter of the manuscript, just before References.

Examples of Data Availability Statements

  • The data that support the findings of this study are available from the corresponding author, [author initials], upon reasonable request.
  • Data availability does not apply to this article as no new data were created or analyzed in this study.
  • The authors confirm that the data supporting the findings of this study are available within the article [and/or its supplementary materials].
  • The data that support the findings of this study are openly available in [repository name] at http://doi.org/[doi], reference number [reference number].
  • The data that support the findings of this study are available from [third party]. Restrictions apply to the availability of these data, which were used under license for this study. Data are available [from the authors / at URL] with the permission of [third party].
  • Raw data were generated at [facility name]. Derived data supporting the findings of this study are available from the corresponding author [initials] on request.
  • The data that support the findings of this study are available on request from the corresponding author, [initials]. The data are not publicly available due to [restrictions e.g., their containing information that could compromise the privacy of research participants].
  • The data that support the findings of this study will be available in [repository name] at [URL/DOI link] following a [3 month] embargo from the date of publication, to allow for the commercialization of research findings.


Please follow the American Psychological Association (APA) 7th Style for references and citations. Please kindly see the guides on this page for citations in the text and reference list.

Citation in the text;

Please ensure that each reference cited in the text is also presented in the reference list. Cite literature in the text in chronological, followed by alphabetical order like these examples “(Nedzvetsky et al., 2012; Palau et al., 2016; Kirici, 2017a, 2017b; Kirici & Atamanalp, 2018)”. If the cited reference is the subject of a sentence, only the date should be given in parentheses. Formatted like this example: “Özdemir (2017)” or “Caf (2017a, 2017b)”.

Parenthetical citation: Kirici, 2021; Perez & Yilmaz, 2020; Atamanalp et al., 2023

Narrative citation: Kirici (2019); Perez & Yilmaz (2020); Atamanalp et al. (2023)

Single author: the author’s name and the year of publication;

Two authors: both authors’ names and the year of publication;

Three or more authors: first author’s name followed by “et al.” and the year of publication.

Citation in the Reference List;

References should be listed first alphabetically and then further sorted chronologically at the end of the article. More than one reference from the same author(s) in the same year must be identified by the letters a, b, c, etc. placed after the year of publication.

The citation of articles, books, multi-author books, and articles published online should conform to the following examples:


Lusher, A. L., Mchugh, M., & Thompson, R. C. (2013). Occurrence of microplastics in the gastrointestinal tract of pelagic and demersal fish from the English Channel. Marine Pollution Bulletin, 67(1-2), 94–99. https://doi.org/10.1016/j.marpolbul.2012.11.028

Viso, A. C., & Marty, J. C. (1993). Fatty acids from 28 marine microalgae. Phytochemistry, 34(6), 1521–1533. https://doi.org/10.1016/S0031-9422(00)90839-2

Kirici, M. (2021).Toxicological effects of metal ions and some pesticides on carbonic anhydrase activity purified from bighead carp (Hypophthalmichthys nobilis) gill tissue. Carpathian Journal of Earth and Environmental Sciences, 16(1), 59 – 65. https://doi.org/10.26471/cjees/2021/016/155

Atamanalp, M., Kırıcı, M., Koktürk, M., Kırıcı, M., Kocaman, E. M., Ucar, A., Parlak, V., Özcan, S., Yanık, T., & Alak, G. (2023). Polyethylene exposure in rainbow trout; suppresses growth and may act as a promoting agent in tissue-based oxidative response, DNA damage and apoptosis. Process Safety and Environmental Protection, 174, 960-970. https://doi.org/10.1016/j.psep.2023.05.005


Preprint Article References:

  • Ideally, use and cite the final, published version of a work. However, if you used the preprint version of a work, cite that version, as shown in the following examples.
  • Preprint versions of articles may or may not be peer-reviewed or maybe the author’s final, peer-reviewed manuscript as accepted for publication.
  • Two common repositories for preprint articles are PsyArXiv and PubMed Central. Follow the same format for other preprint archives.

Zhu, L., Liu, Q., Liu, X., & Zhang, Y. (2021). RSST-ARGM: A Data-Driven Approach to Long-term Sea Surface Temperature Prediction. Researchsquare, Preprint. https://assets.researchsquare.com/files/rs-468686/v1_stamped.pdf

Hampton, S., Rabagliati, H., Sorace, A., & Fletcher-Watson, S. (2017). Autism and bilingualism: A qualitative interview study of parents’ perspectives and experiences. PsyArXiv, Preprint. https://doi.org/10.31234/osf.io/76xfs

Hetland, B., McAndrew, N., Perazzo, J., & Hickman, R. (2018). A qualitative study of factors that influence active family involvement with patient care in the ICU: Survey of critical care nurses. PubMed Central, Preprint. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736422/?report=classic

Articles in non-English languages:

Özcan, M., İspir, Ü., Yonar, M. E., & Şeker, E. (2021). İmidacloprid’e maruz bırakılan Sazan (Cyprinus carpio) yavrularının bazı biyokimyasal özelliklerindeki değişimler [Changes in the some biochemical constituents in fry of common carp (Cyprinus carpio) exposed to ımidacloprid]. Acta Aquatica Turcica, 17(3), 440-444. https://doi.org/10.22392/actaquatr.729034


Mance, G. (1987). Pollution Threat of Heavy Metals in Aquatic Environment. 2nd ed. Elsevier, London.


Landrum, P. F., & Fisher S. W. (1999). Influence of lipids on the bioaccumulation and trophic transfer of organic contaminants in aquatic organisms. In: Arts, M. T., Wainman, B. C., (Eds.), Lipids in Freshwater Ecosystems (pp. 203-234). Springer, New York.

Thesis and Dissertation:

Taysi, M. R. (2019). Gökkuşağı alabalığı (Oncorhynchus mykiss) beyin dokusunda civa klorür toksisitesinin oluşturduğu oksidatif stres ve DNA hasarının belirlenmesi [Determination of oxidative stress and DNA damage caused by mercury chloride toxicity in brain tissue rainbow trout (Oncorhynchus mykiss)]. Ph. D. Thesis, Bingöl University.

Conference Proceedings:

Atamanalp, M., Kırıcı, M., Kırıcı, M., & Beydemir, Ş. (2016). Investigation of in vitro effects of some metal ions on glucose 6-phosphate dehydrogenase which purified from freshwater fish Capoeta umbla kidney. 2nd International Congress on Applied Ichthyology & Aquatic Environment (HydroMediT2016), Messolonghi, Greece, pp. 39-43.

Institution Publication:

EU, (2012). Blue Growth, Opportunities for Marine and Maritime Sustainable Growth. Luxembourg, 13 pp.


HLPE, (2014).Sustainable Fisheries and Aquaculture for Food Security and Nutrition, A Report by the High Level Panel of Experts on Food Security and Nutrition of the Committee on World Food Security. Rome, Italy.

Internet Source:

UN, (2017). Communities of Ocean Action. Retrieved on November 21, 2017, from https://oceanconference.un.org/


Tables should be cited consecutively in the main text and should be submitted as editable text in a Word file. Place footnotes to tables below the table body and indicate them with superscript lowercase letters (or asterisks for significance values and other statistical data). Table numbers should be given consecutively according to their appearance in the text (Table 1, Table 2,...etc). Avoid vertical rules. The data presented in the tables do not duplicate results described elsewhere in the article. No shading line should be used. Use Table format instead of self-produced tables.


All illustrations should be labeled ‘Figure’ and numbered in consecutive Arabic numbers, Figure 1, Figure 2, etc. the text and do not attach captions to the figure. If panels of a figure are labeled (a, b, etc.) use the same case when referring to these panels in the text. Figures, which are recommended for electronic formats such as PNG, JPEG. TIFF (min. 300 dpi) should be also arranged in available dimensions. Place all Figures next to the relevant text in the manuscript. All symbols and abbreviations can be given in the figure caption. Font sizes size should be from 9 to 11 points. The figure caption should be below the figure body.

Units of Measurement:

Units of measurement should be presented simply and concisely using the International System of Units.


Authors are requested to return accepted Proof to the Editor within 5 days of receipt.

Copyright and Permissions:

Authors retain the copyright of their manuscripts, and all open-access articles are distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.