Comparative molecular characterization of Diplozoon species from fishes
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DOI:
https://doi.org/10.5281/zenodo.12800103Keywords:
Diplozoon, Fish parasites, PCR, Phylogenetics, SequencingAbstract
In this article, it was aimed to isolate Diplozoon species from naturally infected carp fish from Elâzığ region and to determine their morphological and microscopic characteristics and molecular identification. As a result of the study, it was observed that parasite isolation was realized in all samples of carp fish sampled. It was observed that the isolated parasites were 33-55/27.3-51.3-51.2/24-45 mm in size. DNA isolation and PCR analysis were performed by selecting the most morphologically and different sized samples. In the preliminary identification analysis by Real-Time PCR, both primer sets and DNA interaction were checked. Then, blast results obtained from the sequence data obtained as a result of PCR analysis performed in the thermal cycler device, it was observed that they showed 98% similarity with Diplozoon paradoxum and the closest similarity with Paradiplozoon homoion and Paradiplozoon skrjabini with a maximum similarity of 96%. Phylogenetic analysis of Diplozoon species using sequence data and Neighbor Joining (NJ) methods showed that they occur in 2 different branches. In line with these results, it was observed that 3 different groups were formed in the parasites with the lowest morphological and microscopic similarity. It is thought that genetic differences are the main reasons why Diplozoon parasite species cause different levels of infestation, virulence and mortality in fish.
Diplozoon species in fish are a common cause of infestation worldwide and diplozoon species are also common in the study area. Intraspecific differences in diplozoon species and changes in the host-parasite relationship lead to differences in virulence and pathogenicity, and as a result, cause diseases and economic losses. Knowing the species differences of parasites regionally has an important place in fish diseases for taking prophylactic measures and treatment processes.
References
Bychowsky, B.E. (1957). Monogenetic Trematodes: Their Systematics and Phylogeny. English translation by Macmillan, A., and Bychowsky, B.E. Academy of Sciences of the USSR, English translation program, Washington, D.C.
Dos Santos, Q. M., & Avenant-Oldewage, A. (2020). Review on the molecular study of the Diplozoidae: analyses of currently available genetic data, what it tells us, and where to go from here. Parasites Vectors, 13, 539. https://doi.org/10.1186/s13071-020-04417-3
Ergens, R. (1969). The suitability of ammonium picrate-glycerin in preparing slides of lower Monogenoidea. Folia Parasitologica, 16(4), 320.
Erk'akan, F., Özuluğ, M., & Bilge, G. (2006). The first record of Diplozoon paradoxum Nordmann, 1832 (Monogenea) from Turkey. Bulletin of the European Association of Fish Pathologists, 26(1), 20-25.
Justine, J. L., Rahmouni, C., and Gey, D. (2011). Diplozoon paradoxum (Monogenea, Diplozoidae) in Africa: redescription and new sites of infection. Acta Parasitologica, 56(3), 275-282.
Karataş, B. (2024). Dietary Cyanus depressus (M. Bieb.) Soják plant extract enhances growth performance, modulates intestinal microbiota, and alters gene expression associated with digestion, antioxidant, stress, and immune responses in rainbow trout (Oncorhynchus mykiss). Aquaculture International, https://doi.org/10.1007/s10499-024-01548-7
Khotenovsky, I. A. (1985). Monogeneans of freshwater fishes and the problem of their evolution. In: Ecology and morphology of parasitic helminths. Moscow, Nauka, p. 48-60.
Levsen, A., Mo, T. A., & Bachmann, L. (2012). Molecular phylogeny and character evolution in the parasitic genus Diplozoon (Platyhelminthes: Monogenea). International Journal for Parasitology, 42(9), 879-889.
Mendoza-Palmero, C. A., Scholz, T., Mendoza-Franco, E. F., & Kuchta, R. (2014). Karyotype and nuclear DNA content of species of the genus Diplozoon (Platyhelminthes: Monogenea). Folia Parasitologica, 61(3), 219-226.
Mizelle, J.D. (1936). Studies on Monogenetic Trematodes. XVIII. The status of the genus Diplozoon Nordmann, 1832, with observations on the structure of the body. Transactions of the American Microscopical Society, 55(1), 37-45.
Önalan, Ş. (2019). Expression differences of stress and immunity genes in rainbow trout (Oncorhynchus mykiss, Walbaum 1792) with different bacterial fish diseases. Israeli Journal of Aquaculture-Bamidgeh, 71. https://doi.org/10.46989/001c.20978
Šimková, A., Plaisance, L., Matějusová, I., Morand, S., & Verneau, O. (2003). Phylogenetic relationships of the genus Diplozoon (Platyhelminthes: Monogenea) based on 28S rDNA sequences. International Journal for Parasitology, 33(2), 167-177.
Svobodová, M., Kašný, M., Baruš, V., Seifertová, M., Matějusová, I., & Šimková, A. (2017). Genetic diversity and population structure of Diplozoon paradoxum (Monogenea: Diplozoidae) from different cyprinid fish species in Czech Republic revealed by microsatellite markers. Parasitology Research, 116(2), 755-766.
Ziętara, M. S., Lumme, J., Pękala, A., & Jusup, M. (2010). DNA extraction from monogenean (platyhelminth) parasite: The first DNA barcoding protocol of gyrodactylid samples preserved in 96% ethanol. Journal of Parasitology, 96(4), 741-743.
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